Discover our scientific papers published in the most prestigious international journals. They are organized in two main parts: Tissue Engineering Expertise and Alternative Methods Development.


Reconstituted epidermis: a novel model for the study of drug metabolism in human epidermis
M.A. Pham (CIRD, France), M.C. Lenoir (CIRD, France), B.A. Bernard (CIRD, France),
J.C. Jamoulle (CIRD, France), J. Magdalou (Centre du Médicament Nancy, Nancy, France),
G. Siest (Centre du Médicament Nancy, Nancy, France), Shroot B. (CIRD, France)
Journal of Investigative Dermatology, 1990 June; 94(6):749-752

EPISKIN: An in vitro model for the evaluation of phototoxicity and sunscreen photoprotective properties
R .Roguet , K. G. Dossou, A. Rougier, C.Cohen, L’Oréal Research & Innovation
Toxicology in Vitro 1994 ;8 (4):669-671

The use of a reconstructed human epidermis Episkin for the assessment of safety and efficacy of cosmetic products
M. Régnier (L’Oréal Research & Innovation), M. J. Staquet (INSERM), D. Schmitt (INSERM), R. Schmidt (L’Oréal Research & Innovation)
Journal of Investigative Dermatology, Volume 109, Issue 4, 1997, Pages 510-512

Reconstructed skin for in vitro cutaneous pharmacotoxicology: Example of metabolic studies
R. Roguet, J. Cotovio, J. Leclaire, L’Oréal Research & Innovation
In Vitro Toxicology: Journal of Molecular and Cellular Toxicology 1997 ;10 (1):3-9

Overview of in vitro cell culture technologies and pharmaco-toxicological applications
H. Schaefer, F.M. Marano, D.J. Benford, J.V. Castell, A.C. Reinhardt, R. Roguet
L'Oréal Research & Innovation; Laboratoire de Cytophysiologie et Toxicologie Cellulaire, Universite Paris VII Denis Diderot, France
Toxicology in vitro 1997 ;11 (5):591-599

UVA exposures of human skin reconstructed in vivo induces apoptosis of dermal fibroblasts: subsequent connective tissue repair and implications in photoaging
F. Bernerd, D. Asselineau, L’Oréal Research & Innovation
Cell Death and Differentiation, 1998 ; 5 : 792-802

Potential cosmetic applications of reconstructed epidermis
C. Duval, R. Schmidt , M. Regnier, L’Oréal Research & Innovation
Toxicology in vitro 1999 ;21 (1):51-58

Reconstructed human skin: alternative models for the safety and efficacy assessment of dermato-cosmetological products
R. Schmidt, C. Duval, M. Régnier, L’Oréal Research & Innovation
Developments in Animal and Veterinary Sciences, 31, 2000

Development of a highly sensitive in vitro phototoxicity assay using the SkinEthic reconstructed human epidermis
M. Rosdy (SkinEthic, France), B. De Wever (SkinEthic, France), F.X. Bernard (BIOAlternatives, France), C. Barrault (BIOAlternatives, France), A. Deguercy (BIOAlternatives, France)
Cell Biology Toxicology 2000 ;16 (6):391-400

Clues to epidermal cancer proneness revealed by reconstruction of DNA repair-deficient xeroderme pigmentosum skin in vitro
F. Bernerd (L’Oréal Research & Innovation), D. Asselineau (L’Oréal Research & Innovation), C. Vioux (L’Oréal Research & Innovation), O. Chevallier-Lagente (Genetic Instability and Cancer Unit, CNRS, France), B. Bouadjar (Department of Dermatology, Centre Hospitalo-Universitaire, Bab-El-Oued, Algeria), A. Sarasin (Genetic Instability and Cancer Unit, CNRS, France), T. Magnaldo (Genetic Instability and Cancer Unit, CNRS, France)
PNAS, 2001, 98/14: 7817-7822

Reconstructed Human skin: effect of solar UV light and use to evaluate efficiency of sunscreens in vitro
F. Bernerd (L’Oréal Research & Innovation), D. Asselineau (L’Oréal Research & Innovation)
Cosmetic Dermatology, 2001

Reconstructed human epidermis equivalents. Characterization and applications in cutaneous pharmacotoxicology
Guest Editors: R. Roguet, M. Bracher, W. Diembeck , M. Ponec
S. Karger Medical and Scientific Publishers, Skin Pharmacology and Applied Skin Physiology, 2002, Supplement

The sun protection factor (SPF) inadequately defines broad spectrum photoprotection: demonstration using skin reconstructed in vitro exposed to UVA, UVBor UV-solar simulated radiation
F. Bernerd, C. Vioux , F. Lejeune , D. Asselineau, L’Oréal Research & Innovation
European Journal of Dermatology, 2003;13 (3):242-9

Gene expression profiles of three different models of reconstructed human epidermis and classical cultures of keratinocytes using cDNA arrays
B. Méhul (L’Oréal Research), D. Asselineau (L’Oréal Research), D. Bernard (L’Oréal Research), J. Leclaire (L’Oréal Research), M. Régnier (L’Oréal Research), R. Schmidt (L’Oréal Research), F. Bernerd (L’Oréal Research)
Archives of Dermatology Research, 2004; 296: 145-156

Iron chelation can modulate UVA-induced lipid peroxidation and ferritin expression in human reconstructed epidermis
J.B. Galey, P. Portes, S. Seite, E. Popovic, M.P. Verdier, A. Fourtanier, R. Roguet, C. Cohen
Photodermatology Photoimmunology Photomedecine 2004 Feb ;20 (1):47-52

Development of genotoxicity test procedures with Episkin®, a reconstructed human skin model: Towards new tools for in vitro risk assessment of dermally applied compounds?
N. Flamanda, L. Marrota, J.P. Belaidia, L. Bouroufa, E. Dourilleb, M. Feltesa, and J.R. Meunier, L’Oréal Research & Innovation
Mutation Research/Genetic Toxicology and Environmental Mutagenesis Volume 606, Issues 1-2, 14 July 2006, Pages 39-51

Reconstructed human skin: From photodamage to sunscreen protection and anti-aging molecules
C. Vioux-Chagnoleau, F. Lejeune, J. Sok, C. Pierrard, C. Marionnet, F. Bernerd, L’Oréal Research & Innovation
Journal of Dermatological Science Suplement (2006), 2, S1-S12

Morphogenesis of dermal-epidermal junction in a model of reconstructed skin: beneficial effects of vitamin C.
C. Marionnet C. Vioux-Chagnoleau, C. Pierrard, J. Sok, D. Asselineau, F. Bernerd
L’Oréal Research & Innovation
Experimental Dermatology 2006;15(8):625-33.

Improvement of the dermal epidermal junction in human reconstructed skin by a new c-xylopyranoside derivative.
J. Sok, N. Pineau, M. Dalko-Csiba, L. Breton, F. Bernerd, L’Oréal Research & Innovation
European Journal of Dermatology, 2008 May-Jun;18(3):297-302. Epub 2008 May 13

An organotupic model of skin to study photodamage and photoprotection in vitro
F. Bernerd, D. Asselineau, L’Oréal Research & Innovation
Journal of American Academy of Dermatology, 2008, vol. 58, number 5

Ultraviolet A within sunlight induces mutations in the epidermal basal layer of engineered human skin.
X.X. Huang, F. Bernerd, G.M. Halliday
American Journal of Pathology, 2009 Apr;174(4):1534-43. Epub 2009 Mar 5.

A catch-up validation study on reconstructed human epidermis (SkinEthic™ RHE) for full replacement of the Draize skin irritation test
N. Alépée (L’Oréal), C. Tornier (SkinEthic Laboratories), C. Robert (Coty), C. Amsellem (Episkin SNC), M.H. Roux (Oroxcell) , O. Doucet (Coty), J. Pachot (Oroxcell), M. Méloni (Vitroscreen), A. de Brugerolle de Fraissinette (SkinEthic Laboratories)
Toxicology in vitro, 2010, 24, 257-266


Evaluation of the effects of cosmetic or dermo-pharmaceutical products on cutaneous energy metabolism using the Episkin model of reconstructed epidermis
P. Buche, L. Violin , P. Girard, Dermscan, France
Cell Biology and Toxicology, 1994 ;10 (5-6):381-5

The use of in vitro reconstituted human skin in dermotoxicity testing
R. Roguet, K. G. Dossou, A. Rougier, M. Regnier,C. Cohen, L’Oréal Research & Innovation
Toxicology in Vitro 1994 ;8 (4):635-639

Use of skin cell cultures for in vitro assessment of corrosion and cutaneous irritancy
R. Roguet, L’Oréal Research & Innovation
Cell Biology and Toxicology, 1999 ;15 (1):63-75

Use of a standardized reconstructed epidermis kit to assess in vitro the tolerance and the efficacy of cosmetics
R. Roguet, C. Cohen, J. Leclaire, L’Oréal Research & Innovation
Tessonneaud E., Gagne C., Teissier M.H., Episkin, France
International Journal of Cosmetic Science, 22 : 409-419 ; 2000

Non-animal testing strategies for assessment of the skin corrosion and skin irritation potential of ingredients and finished products
M. K. Robinson (Human & Environmental Safety Division, The Procter & Gamble), J.H. Fentem ( SEAC, Unilever Colworth Laboratory, Sharnbrook, UK), A. de Brugerolle de Fraissinette (Preclinical Safety/Toxicology-Pathology Department, Novartis), E. Whittle (SEAC, Unilever Colworth Laboratory, Sharnbrook, UK), M. Ponec M. (Department of Dermatology, Leiden University Medical Center, Leiden, The Netherlands), C. Cohen (L’Oréal Research & Innovation)
Food and Chemical Toxicology, 2002 ;40 (5):573-92

The in vitro Acute Skin Irritation of Chemicals: Optimisation of the Episkin Prediction Model within the Framework of the ECVAM Validation Process
J. Cotovio, M.H. Grandidier, P. Portes, R. Roguet, and G. Rubinstenn
L’Oréal Research & Innovation
ATLA 33, 329-349, 2005

In vitro skin irritation testing on reconstructed human epidermis : reproducibility for 50 chemicals tested with two protocols
C. Tornier, M. Rosdy, H.I. Maibach
SkinEthic Laboratories, France
UCSF, San Francisco, USA
Toxicology in Vitro, 2006, 20, 401-416

Prevalidation of a new in vitro reconstituted human cornea model to assess the eye irritating potential of chemicals
B. De Wever (SkinEthic Laboratories, France), F. Van Goethem (Johnson & Johnson Pharmaceutical Research and Development, Belgium) , E. Adriaens (Laboratory of Pharmaceutical Technology, Belgium) , N. Alépée (Pfizer Global R&D, France), F. Straube (BMD, Novartis Pharma Switzerland), M. Cappadoro (SkinEthic Laboratories, France), S. Catoire, E. Hansen (Johnson & Johnson Pharmaceutical Research and Development, Belgium), A. Wolf (BMD, Novartis Pharma, Switzerland), P. Vanparys (Johnson & Johnson Pharmaceutical Research and Development, Belgium)
Toxicology In Vitro. 2006 Feb;20(1):1-17

Activation of U937 Cells by contact Sensitizers: CD86 Expression is independent of Apoptosis
N. Ade, S. Teissier, L’Oréal Research & Innovation
Journal of Immunotoxicology, 3:189-197, 2006

The ECVAM international validation study on in vitro tests for acute skin irritation: report on the validity of the EPISKIN and EpiDerm assays and on the Skin Integrity Function Test
H. Spielmann, S. Hoffmann, M. Liebsch, P. Botham, J.H. Fentem, C. Eskes, R. Roguet, J. Cotovio, T. Cole, A. Worth, J. Heylings, P. Jones, C. Robles, H. Kandárová, A. Gamer, M. Remmele, R. Curren,H. Raabe, A. Cockshott, I. Gerner, V. Zuang
Alternatives to Laboratory Animals ATLA 2007 Dec;35(6):559-601

The EpiSkin phototoxicity assay (EPA): development of an in vitro tiered strategy using 17 reference chemicals to predict phototoxic potency
D. Lelièvre, P. Justine, F. Christiaens, N. Bonaventure, J. Coutet, L. Marrot, J. Cotovio, L’Oréal Research & Innovation
Toxicology In Vitro. 2007 Sep;21(6):977-95. Epub 2007 May 1

How to reduce false positive results when undertaking in vitro genotoxicity testing and thus avoid unnecessary follow-up animal tests: Report of an ECVAM Workshop
D. Kirkland1, S. Pfuhler2 , D. Tweats3, M. Aardema4, R. Corvi5 , F. Darroudi6, A. Elhajouji7 , H. Glatt8, P. Hastwell9, M. Hayashi10, P. Kasper11, S. Kirchner12, A. Lynch9 D. Marzin13, D. Maurici5, J.R. Meunier14, L. Mueller12, G. Nohynek14, J. Parry3, E. Parry3, V. Thybaud15, R. Tice16 , J. van Benthem17, P. Vanparys18, and P. White19
1 Covance Laboratories Ltd., Otley Road, Harrogate, HG3 1PY, United Kingdom
2 Procter and Gamble, Cosmital SA, Rte de Chesalles 26, CH-1723 Marly, Switzerland
3 Medical School, University of Wales Swansea, Singleton Park, Swansea, SA2 8PP, United Kingdom
4 Procter and Gamble Co., Miami Valley Innovation Center, 11810 East Miami River Road, Cincinnati, OH 45239-8707, United States
5 European Centre for the Validation of Alternative Methods (ECVAM), Institute for Health and Consumer Protection (IHCP), European Commission-Joint Research Centre, Via E. Fermi 1, I-21020 Ispra, (Va), Italy
6 Department of Toxicogenetics, Leiden University Medical Centre, Einthovenweg 20, 2300-RC Leiden, Netherlands
7 Novartis Pharma AG, MUT-2881.5.38, CH-4002 Basel, Switzerland
8 German Institute of Human Nutrition (DIfE) Potsdam-Rehbruecke, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany
9 GlaxoSmithKline R and D, Park Road, Ware, Herts SG12 0DP, United Kingdom
10 Division of Genetics and Mutagenesis, NIHS, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo, 158-8501, Japan
11 BfArM, Genetic Toxicology, Kurt-George-Kiesinger Allee 3, D-53175 Bonn, Germany
12 Non-clinical Drug Safety, F. Hoffmann-La Roche, PRBN-T, Bldg. 73-311b, CH-4070 Basel, Switzerland
13 Institut Pasteur de Lille, 1 rue de Profeseur Calmette, BP 245, 59019 Lille Cedex, France
14 L'Oréal Research & Innovation, France
15 Sanofi-Aventis, 13 Quai Jules Guesde, BP 14, Vitry sur Seine, 94403 Cedex, France
16 NICEATM, NIEHS, Mail Code EC-17, P.O. Box 12233, Research Triangle Park, NC 27709, United States
17 National Institute of Public Health and the Environment, Antonie Leeuwenhoeklaan 9, P.O.B. 1, 3720 BA Bilthoven, Netherlands
18 Johnson and Johnson Pharmaceutical Research and Development, Turnhoutseweg 30, B-2340 Beerse, Belgium
19 Mutagenesis Section, Safe Environments Programme, Health Canada, Tunney's Pasture 0803A, Ottawa, Ontario K1A O12, Canada
Mutation Research – Genetic Toxicology and Environmental Mutagenesis 2007, 628(1), pp.31-55

In vitro acute skin irritancy of chemicals using the validated EPISKIN model in a tiered strategy. Results and performances with 184 cosmetic ingredients
J. Cotovio, M. H. Grandidier, D. Lelièvre, R. Roguet, E. Tinois-Tessonneaud, and J. Leclaire AATEX 14, Special Issue, 351-358 WC6, August 2007, Japan
2008, Japanese Society for Alternatives to Animal Experiments

Expression profiles of phases 1 and 2 metabolizing enzymes in human skin and the reconstructed skin models Episkin™ and full thickness model from Episkin™
V. Luu-The (Quebec University Hospital Research Center and Laval University), D. Duche (L’Oréal Research & Innovation), C. Ferraris (L’Oréal Research & Innovation), J.R. Meunier (L’Oréal Research & Innovation), J. Leclaire (L’Oréal Research & Innovation), F. Labrie (Quebec University Hospital Research Center and Laval University)
Journal of Steroid Biochemistry & Molecular Biology, 2009, 116, 178-186

Prediction of chemical absorption into and through the skin from cosmetic and dermatological formulations
S. Grégoire, C. Ribaud, F. Benech, J.R.Meunier, A. Garrigues-Mazert, and R.H. Guy*
L’Oréal Research & Innovation, *Department of Pharmacy and Pharmacology, University of bath, United Kingdom
British Journal of Dermatology, 2009, 160, pp80-91

Assessment of the optimized SkinEthic Reconstructed Human Epidermis (RHE) 42 bis skin Irritation protocol over 39 test substances
C. Tornier1, C. Amsellem2 , A. de Brugerolle de Fraissinette1, N. Alépée3
1 SkinEthic Laboratories, France
2 Episkin, France
3 L’Oréal Research & Innovation, France
Toxicology in Vitro 24 (2010) 245-256

The fish embryo toxicity test as an animal alternative method in hazard and risk assessment and scientific research
M.R. Embry (ILSI), S.E. Belanger (Procter & Gamble), T.A. Braunbeck (University of Heidelberg), M. Galay-Burgos (European Centre for Ecotoxicology and Toxicology of Chemicals), M. Halder (European commission), D.E. Hinton (Duke University), M.A. Léonard (L’Oréal Recherche), A. Lillicrap (AstraZeneca), T. Norberg-King (US EPA), G. Whale (Shell Global Solutions)
Aquatic Toxicology, 2010, 97, 79-87

In vitro assessment of eye irritancy using the Reconstructed Human Corneal Epithelial SkinEthic™ HEC model : Application to 435 substances from consumer products industry
J. Cotovio (L’Oréal Research & Innovation), M.H. Grandidier (L’Oréal Research & Innovation), D. Lelièvre (L’Oréal Research & Innovation), C. Bremond (Episkin SNC), C. Amsellem (Episkin SNC), S. Maloug (L’Oréal Research & Innovation), J.M. Ovigne (L’Oréal Research & Innovation), S. Loisel-Joubert (L’Oréal Research & Innovation), A. Van Der Lee (L’Oréal Research & Innovation), A.M. Minondo (L’Oréal Research & Innovation), C. Capallere (SkinEthic Laboratories), B. Bertino (SkinEthic Laboratories), N. Alépée (L’Oréal Research & Innovation), E. Tinois-Tessonneaud (Episkin SNC), A. de Brugerolle de Fraissinette (SkinEthic Laboratories), J.R. Meunier (L’Oréal Research & Innovation), J. Leclaire (L’Oréal Research & Innovation)
Toxicology in vitro, 2010, 24, 523-537

Identifying and characterizing chemical skin sensitizers without animal testing : Colipa’s research and method development program
P. Aeby (Colipa), T. Ashikaga (Shiseido Research Centre), S. Bessou-Touya (Pierre Fabre), A. Schepky (Beiersdorf), F. Gerberick (Procter & Gamble), P. Kern (Procter & Gamble), M. Marrec-Fairley (Colipa), G. Maxwell (Unilever), J.M. Ovigne (L’Oréal), H. Sakaguchi (Kao Corporation), K. Reisinger (Henkel AG & Co), M. Tailhardat (LVMH Recherche), S. Martinozzi-Teissier (L’Oréal), P. Winkler (Johnson & Johnson)
Toxicology in vitro, 2010, 24, 1465-1473

A proposed eye irritation testing strategy to reduce and replace in vivo studies using Bottom-Up and Top-Down approaches
L. Scott (Procter & Gamble), C. Eskes (ECVAM), S. Hoffmann (ECVAM), E. Adriaens (Gent University), N. Alepée (L’Oréal), M. Bufo (Res Pharm Srl), R. Clothier (FRAME at the School of Biomedical Sciences), D. Facchini (INT.E.GRA), C. Faller (Cosmital SA), R. Guest (Harlan Laboratories Ltd), J. Harbell (Mary Kay Inc), T. Hartung (ECVAM), H. Kamp (BASF), B. Le Varlet (LVMH Parfums et Cosmétiques R&D), M. Meloni (Vitroscreen), P. McNamee (Procter & Gamble), R. Osborne (Procter & Gamble), W. Pape (Beiersdorf), U. Pfannenbecker (Beiersdorf), M. Prinsen (TNO), C. Seaman (GlaxoSmithKline Ware), H. Spielmann (BfR-ZEBET), W. Stokes (National Institute of Environmental Health Siences , K. Trouba (Procter & Gamble), C. Van den Berghe (L’Oréal), F. Van Goethem (Johnson & Johnson), M. Vassallo (Res Pharm Srl), P. Vinardell (University of Barcelona), V. Zuang (ECVAM)
Toxicology in vitro, 2010, 24, 1-9

Prospective Validation Study of Reconstructed Human Tissue Models for Eye irritation Testing
S.J. Freeman1, N. Alépée2, J. Barroso3, T. Cole3, A. Compagnoni3, C. Rubingh4, C. Eskes5, J. Lammers4, P. McNamee6, U. Pjannenbecker7, and V. Zuang3
1 Farino Consulting, UK, Chair VMG3, 2 L’Oréal Research & Innovation, France 3 ECVAM, Italy 4 TNO, The Netherlands 5 Independent Consultant 6 Colipa Berlgium, 7 Beiersdorf, Germany ALTEX 27, Special issue 2010

The reconstructed skin micronucleus assay (RSMN) in EpiDerm™: Detailed protocol and harmonized scoring atlas
E.L. Dahl1, R. Curren1 , B.C. Barnett 2, 7, Z. Khambatta 2, K. Reisinger 3, G. Ouédraogo4, B. Faquet4 , A. C. Ginestet4 , G. Mun 1, N.J. Hewitt 5, G. Carr 2,S. Pfuhler3, and M.J. Aardema6
1 Institute for In Vitro Sciences, Inc., Gaithersburg MD, United States
2 Procter and Gamble, Miami Valley Laboratories, Cincinnati 45253, United States
3 Henkel AG and Co KGaA, Düsseldorf, Germany
4 L'Oréal Research & Innovation, France
5 SWS, Erzhausen, Germany
6 Marilyn Aardema Consulting, LLC, 5315 Oakbrook Dr., Fairfield, OH, United States
7 ATL, Procter and Gamble, Miami Valley Laboratories, Cincinnati, 45253, United States
Mutation Research – Genetic Toxicology and Environmental Mutagenesis, 2011, 720 (1-2), pp.42-52

J. Cotovio, Predictive Models & Methods

Tissue Engineering & Alternatives Methods

Agrostat 2016 is the 14th Symposium on Statistical Methods for the Food Industry (Lausanne, Switzerland 21st-24th March 2016). L’Oréal Research & Innovation was proud to sponsor this congress.

Discover our contributions for Sensometrics, Chemometrics and Risk & Process oral sessions.